Latest Flintbox Projects

Nkx3.1; Pten mutant mice

2010-09-07 11:35:17

Nkx3.1 homeobox gene has restricted expression in the prostate and essential role in prostate differentiation and function. PTEN represents a frequent target of mutational inactivation in human cancers, and Pten heterozygous mutant mice develop cancers or dysplasias of multiple tissues including prostate. Loss of function of Nkx3.1 and Pten cooperate in prostate cancer progression. These mice are compound mutants in both Nkx3.1 and Pten, leading to synergistic activation of Akt in the prostate suggesting that Akt deregulation is a critical event in prostate carcinogenesis.

Rd114

2010-09-07 11:34:34

These cells are a stable RD114-expressing packaging line capable of high-level transduction of human CD34+ cells. Viral particles from this cell line were concentrated up to 100-fold (up to107 viral particles/ml) by ultracentrifugation. Human hematopoietic progenitors from cord blood and sickle cell CD34+ cells were efficiently transduced with a NeoR-containing vector after a single exposure to concentrated RD114-pseudotyped virus. produced from this cell line.

Retinol Binding Protein- 4 mice

2010-09-07 11:33:52

Reduction of dietary vitamin A intake in RBP knockout mice (RBP-/-) rapidly reproduces a vitamin A-deficient (VAD) status, making this strain a model to study the role of vitamin A in specific organs and tissues. These mouse can be used a model to study the role of vitamin A in specific organs and tissues.

TRE2-Axin-GFP mice

2010-09-07 11:33:11

Axin is a component of the canonical Wnt pathway that negatively regulates signal transduction by promoting degradation of Î²-catenin.

Rosa26-YFP mice

2010-09-07 11:32:23

This mouse strain expresses YFP from the ubiquitously ROSA26 locus promoter, after a transcriptional termination sequence, flanked by loxP sites, has been removed by Cre recombinase activity.

Rosa26-CFP mice

2010-09-07 11:31:18

This mouse strain expresses CFP from the ubiquitously ROSA26 locus promoter, after a transcriptional termination sequence, flanked by loxP sites, has been removed by Cre recombinase activity.

Hox7-EGFP mice

2010-09-07 11:30:31

In these transgenic mice, GFP is expressed in the ureteric bud under the control of the Hoxb7 promoter. In these mice, GFP is expressed in every branch of the ureteric bud throughout renal development, and in its derivative epithelia in the adult kidney.

Axin2-d2EGFP mice

2010-09-07 11:28:29

A 5.6-kb genomic sequence from the Axin2 promoter was sufficient to direct the tissue-specific expression of d2EGFP in transgenic embryos, consistent with a role for the Tcf/LEF sites and surrounding conserved sequences in the in vivo expression pattern of Axin2.

Diffuse Large B- Cell Lymphoma (DLBCL)-BCL6 mouse

2010-09-07 10:54:50

Diffuse large B cell lymphomas (DLBCL) derive from germinal center (GC) B cells and display chromosomal alterations deregulating the expression of BCL6, a transcriptional repressor required for GC formation. To investigate the role of BCL6 in DLBCL pathogenesis, we have engineered mice that express BCL6 constitutively in B cells by mimicking a chromosomal translocation found in human DLBCL. These mice display increased GC formation and perturbed post-GC differentiation characterized by a decreased number of post-isotype switch plasma cells. Subsequently, these mice develop a lymphoproliferative syndrome that culminates with the development of lymphomas displaying features typical of human DLBCL.

LPL-GPI mice

2010-09-07 10:54:14